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Amino acids 473V and 598P of PB1 from an avian-origin influenza A virus contribute to polymerase activity, especially in mammalian cells.

Identifieur interne : 000982 ( Main/Exploration ); précédent : 000981; suivant : 000983

Amino acids 473V and 598P of PB1 from an avian-origin influenza A virus contribute to polymerase activity, especially in mammalian cells.

Auteurs : Chen Xu [République populaire de Chine] ; Wei-Bin Hu [République populaire de Chine] ; Ke Xu [République populaire de Chine] ; Yun-Xia He [République populaire de Chine] ; Tong-Yan Wang [République populaire de Chine] ; Ze Chen [République populaire de Chine] ; Tian-Xian Li [République populaire de Chine] ; Jin-Hua Liu [République populaire de Chine] ; Philippe Buchy [Cambodge] ; Bing Sun [République populaire de Chine]

Source :

RBID : Hal:pasteur-00723699

Abstract

It has been reported that the avian-origin influenza A virus PB1 protein (avian PB1) enhances influenza A virus polymerase activity in mammalian cells when it replaces the human-origin PB1 protein (human PB1). Characterization of the amino acid residues that contribute to this enhancement is needed. In this study, it was found that PB1 from an avian-origin influenza A virus [A/Cambodia/P0322095/2005, H5N1 (Cam)] could enhance the polymerase activity of an attenuated human isolated virus, A/WSN/33, carrying the PB2 K627E mutation (WSN627E) in vitro. Furthermore, 473V and 598P in the Cam PB1 were identified as the residues responsible for this enhanced activity. The results from recombinant virus experiments demonstrated the contribution of PB1 amino acids 473V and 598P to polymerase activity in mammalian cells and in mice. Interestingly, 473V is conserved in pH1N1 viruses from the 2009 pandemic. Substitution of 473V by leucine in pH1N1 PB1 led to a decreased viral polymerase activity and a lower growth rate in mammalian cells, suggesting that the PB1 473V also plays a role in maintaining efficient virus replication of the pH1N1 virus. Thus, it was concluded that two amino acids in avian-origin PB1, 473V and 598P, contribute to the polymerase activity of the H5N1 virus, especially in mammalian cells, and that 473V in PB1 also contributes to efficient replication of the pH1N1 strain.


Url:
DOI: 10.1099/vir.0.036434-0


Affiliations:

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<name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name>
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<idno type="DOI">10.1099/vir.0.036434-0</idno>
<series>
<title level="j">Journal of General Virology</title>
<idno type="ISSN">0022-1317</idno>
<imprint>
<date type="datePub">2012-03</date>
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<p>It has been reported that the avian-origin influenza A virus PB1 protein (avian PB1) enhances influenza A virus polymerase activity in mammalian cells when it replaces the human-origin PB1 protein (human PB1). Characterization of the amino acid residues that contribute to this enhancement is needed. In this study, it was found that PB1 from an avian-origin influenza A virus [A/Cambodia/P0322095/2005, H5N1 (Cam)] could enhance the polymerase activity of an attenuated human isolated virus, A/WSN/33, carrying the PB2 K627E mutation (WSN627E) in vitro. Furthermore, 473V and 598P in the Cam PB1 were identified as the residues responsible for this enhanced activity. The results from recombinant virus experiments demonstrated the contribution of PB1 amino acids 473V and 598P to polymerase activity in mammalian cells and in mice. Interestingly, 473V is conserved in pH1N1 viruses from the 2009 pandemic. Substitution of 473V by leucine in pH1N1 PB1 led to a decreased viral polymerase activity and a lower growth rate in mammalian cells, suggesting that the PB1 473V also plays a role in maintaining efficient virus replication of the pH1N1 virus. Thus, it was concluded that two amino acids in avian-origin PB1, 473V and 598P, contribute to the polymerase activity of the H5N1 virus, especially in mammalian cells, and that 473V in PB1 also contributes to efficient replication of the pH1N1 strain.</p>
</div>
</front>
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<name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name>
<name sortKey="He, Yun Xia" sort="He, Yun Xia" uniqKey="He Y" first="Yun-Xia" last="He">Yun-Xia He</name>
<name sortKey="Hu, Wei Bin" sort="Hu, Wei Bin" uniqKey="Hu W" first="Wei-Bin" last="Hu">Wei-Bin Hu</name>
<name sortKey="Li, Tian Xian" sort="Li, Tian Xian" uniqKey="Li T" first="Tian-Xian" last="Li">Tian-Xian Li</name>
<name sortKey="Liu, Jin Hua" sort="Liu, Jin Hua" uniqKey="Liu J" first="Jin-Hua" last="Liu">Jin-Hua Liu</name>
<name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name>
<name sortKey="Wang, Tong Yan" sort="Wang, Tong Yan" uniqKey="Wang T" first="Tong-Yan" last="Wang">Tong-Yan Wang</name>
<name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name>
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<name sortKey="Buchy, Philippe" sort="Buchy, Philippe" uniqKey="Buchy P" first="Philippe" last="Buchy">Philippe Buchy</name>
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